The scientific article analyzes the methods of determining the gender of performers of handwritten texts, which are used in expert work in today’s conditions. Based on the research of legal acts, scientific positions of leading scientists on this issue, an attempt was made to provide proposals for the latest developments in conducting the specified non-identifying research. Features of preparation of materials for classification of manuscripts into female and male are highlighted. On the basis of a thorough study of the conclusions of court experts, the typical signs of handwriting, which were most often used to determine the gender of the performer, were indicated. Attention is drawn to the factors that influence the change in handwriting, including psychological factors. The need to develop research methods for texts of different scope and degree of elaboration is emphasized. The need to update the outdated methods of determining the sex of the performer in order to eliminate mistakes and errors in expert research has been proven. At the same time, it is emphasized that the existing methods developed on the basis of experimental studies of the Russian alphabet, which did not take into account the development of subsystems of Ukrainian spelling (graphics, orthography, punctuation), historical changes, etc., cannot be objective and be used when conducting forensic handwriting examinations. The conducted scientific research is accompanied by statistical data obtained in the process of surveying law enforcement officers and analyzing expert opinions, as well as examples from expert practice.